Rat GDNF (Glial Cell Line Derived Neurotrophic Factor) CLIA Kit
Detection Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Product Details
Properties
Assay type
Sandwich-CLIA
Format
96T
Assay time
3.5h
Detection range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Sample type &Sample volume
serum, plasma and other biological fluids; 100L
Specificity
This kit recognizes Rat GDNF in samples. No significant cross-reactivity or interference between Rat GDNF and analogues was observed.
Reproducibility
Both intra-CV and inter-CV are < 15%.
Application
This CLIA kit applies to the in vitro quantitative determination of Rat GDNF concentrations in serum, plasma and other biological fluids.
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDNF. You can calculate the concentration of Rat GDNF in the samples by comparing the RLU value of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 2-8 for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Item
Specifications
Storage
Micro CLIA Plate(Dismountable)
96T: 8 wells 12 strips 48T: 8 wells 6 strips
-20, 12 months
Reference Standard
96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100)
96T: 1 vial, 120 L 48T: 1 vial, 60 L
Concentrated HRP Conjugate (100)
96T: 1 vial, 120 L 48T: 1 vial, 60 L
-20(Protect from light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
Concentrated Wash Buffer (25)
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
2-8(Protect from light)
Substrate Reagent B
1 vial, 5 mL
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GDNF were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GDNF were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
54.07
121.99
408.12
54.44
128.92
436.94
Standard deviation
4.46
13.91
45.22
6.07
13.25
39.41
CV (%)
8.25
11.40
11.08
11.15
10.28
9.02
Recovery
The recovery of Rat GDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
84-99
91
EDTA plasma(n=8)
86-98
93
Cell culture media(n=8)
94-110
101
Target Information
Synonyms
ATF, ATF1, ATF2, HFB1-GDNF, HSCR3
Research Area
Cancer, Developmental biology, Neuroscience
Assay Procedures
1.Add 100 L of standard or sample to each well. Incubate for 90 min at 37.
2.Remove the liquid.
3.Add 100 L Biotinylated Detection Ab. Incubate 1 hour at 37. Aspirate and wash 3 times.
4.Add 100 L HRP Conjugate. Incubate 30 min at 37. Aspirate and wash 5 times.
5.Add 100 L Substrate Mixture Solution. Incubate for 5 min at 37.
6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.
Express delivery and return policies
Free shipping on orders over $30
Processing time: 1-2 Days
Shipping time: 3-5 Days(According to the logistics method and delivery address)
Items can be returned or exchanged within 30 days !
